Rederivation from live mice/fresh embryos

The rederivation of mouse lines through embryo transfer is used to establish Specific Pathogen Free (SPF) mice from mice of any compromised health status. Pre-implantation embryos are transferred into pseudopregnant females in an SPF facility.

Rederivation from cryopreserved/thawed embryos

Many mouse strains/lines are cryopreserved as embryos to preserve the line without maintenance breeding, to serve as a backup against a potential pathogen outbreak and to transfer strains between facilities/institutions.

When lines need to be rederived from cryopreserved stocks, embryos are thawed, cultured and then transferred in to pseudopregnant females.

Rederivation section

Rederivation from cryopreserved embryos
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8-cell embryo suitable for rederivation

Introduction

Many mouse strains/lines are cryopreserved as embryos to preserve the line without maintenance breeding, to serve as a backup against a potential pathogen outbreak and to transfer strains between facilities/institutions.

When lines need to be rederived from cryopreserved stocks, embryos are thawed, cultured and then transferred into pseudopregnant females.

Costs

Internal Clients: $418.95/round
External Clients: $773.96/round

This includes:

  • Embryo thaw (TASQ will thaw a series of straws until approximately 40 embryos are obtained to perform embryo transfer)
  • Embryo culture
  • Embryo transfer into pseudopregnant females
  • Agistment of pseudopregnant female and pups until the resulting progeny are weaned. (Agistment is the weekly charge applied by the anmal facilities for the basic care of the animal.  Please contact TASQ for pricing.)
  • Tissue sampling of rederived progeny.
 

Additional Costs

  • Courier or shipping costs of cryopreserved embryos to TASQ - if applicable
  • Animal agistment of progeny once weaned
  • Courier costs of mice to receiving facility/institution
  • Any additional health screens

How to Start

  1. Download, complete and return the Thaw Cryopreserved registration form to TASQ
  2. TASQ will then send a health report of the UQBR animal facility that the line will be rederived into, to the receiving animal facility manager.
  3. Once the UQBR health report has been accepted, TASQ will organize the date for thaw/rederive.

Timeline

Week 1

  • Embryo thaw, culture and embryo transfer

Week 4

  • Progeny are due. Client will be notified of births.

Week 5

  • Tissue samples will be taken for genotyping and sent to Client.

Week 7

  • Progeny will be weaned.

When genotyping results are returned to TASQ the receiving animal facility manager will be contacted to organize a shipment date.

Additional Notes

  • TASQ can not guarantee that 100% of the cryopreserved embryos will survive the thaw process.
  • TASQ will embryo transfer all viable embryos.
  • TASQ cannot guarantee 100% of embryo transfers will result in live births.
  • Registration form must be returned to TASQ prior to commencement of service.
  • A current AEC number and OGTR number must be supplied prior to commencement of service.
Rederivation from fresh embryos
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Single-two cell image

Introduction

The rederivation of mouse lines through embryo transfer is used to establish Specific Pathogen Free (SPF) mice from mice of any compromised health status. Pre-implantation embryos are transferred into pseudopregnant females in an SPF facility.

The efficiency of a rederivation will depend on the individual strain, especially the robustness and fecundity of the strain. Usually inbred mice will yield less viable embryos than a hybrid/outbred strain.

Embryos used for rederivation are produced via IVF.

Excess embryos from the IVF after the embryo transfers will be cryopreserved.

Mice Needs

  • The number of times TASQ will need to perform a rederivation will vary greatly between strains. The major factors in the success of the rederivation are the age of the females, the strain background and the fertility of the males.
  • Females to be used must be aged between 8 and 16 weeks. At this age the females are more receptive to the superovulation regime, therefore producing more viable eggs.
  • Males must be of proven fertility.
  • For one round of IVF, a minimum of 8-10 females  and 2 males are required.
  • If these numbers are not available, please contact TASQ for consultation for a different strategy.
  • Wild type females can be purchased on the researcher’s behalf at an additional cost.

Costs

Internal Clients: $948.15
External Clients: $1455.30

This includes:

  • Superovulation of female mice
  • Egg collection
  • Sperm collection and prep
  • IVF
  • Embryo culture
  • Embryo transfer of up to 60 embryos into pseudopregnant females, within the SPF facility
  • Cryopreservatoin and storage of excess embryos
  • Agistment of pseudopregnant females and progeny until the resulting progeny are weaned. Agistment is the weekly charge applied by the animal facilities for the basic care of the animal (contact TASQ for charges).

Additional Costs

  • Courier costs for shipping mice to a UQBR animal house (if applicable).
  • Agistment of studs and donors.
  • Any wild type females needed. This will depend on strain required.
  • Tissue sampling of rederived progeny (contact TASQ for charges).
  • Shipping costs of tissue samples to client
  • Courier costs of mice to receiving facility/institution (if applicable)
  • Any additional health screens.

How to Start

  • Contact TASQ and arrange health reports to be sent to TASQ (if applicable)
  • Download the relevant IVF rederivation registration form (internal client or external client) and return to TASQ
  • TASQ will send a health report of the UQBR animal facility that the line will be rederived into, to the receiving animal facility manager.
  • Once the UQBR health report has been accepted, TASQ will organize the shipment of the mice to the appropriate UQBR facility.
  • The rederivation will be scheduled and the Client will be sent notification of that date.

Timeline

Week 1             Superovulation regime begins

Week 2             IVF rederivation and embryo transfer

Week 4             Progeny are due. Client will be notified of births

Week 5             Tissue samples will be taken for genotyping

Week 7             Progeny will be weaned and are then ready to be shipped/moved to the relevant facility/institution.

Additional Notes

  • A current AEC certificate and OGTR number must be supplied prior to commencement of service.
  • Registration form must be returned to TASQ prior to commencement of service.
  • TASQ cannot guarantee that 100% of IVF rederivations will result in live births.
  • TASQ will transfer up to 60 embryos per round of IVF but cannot guarantee 100% of embryo transfers will result in live births.
  • TASQ requires that all rederived mice be genotyped. This will be confirmation that progeny are of the expected genotype.
  • TASQ will not cull donor or stud stocks held at UQ until request by the client.
  • In the event that no live births results from the first round of IVF, TASQ will notify the client and a second round of rederivation will be scheduled. Each subsequent rederivation attempt is classified as a “new round of rederivation” and will therefore accrue additional charges.
  • Excess embryos generated from the IVF (and after the embryo transfers) will be cryopreserved. These embryos will be stored at UQ or shipped to a receiving institution at the client’s request.
  • All rederived progeny remain intellectual property of the Client.